Hey all, I've been thinking a lot recently about how much reagent quality really matters. I mean, obviously, it matters, but where do you draw the line between "good enough" and "absolutely perfect" when you're balancing cost and the need to get results! I've noticed that sometimes, using a slightly cheaper reagent doesn't seem to make a huge difference in my experiments. But then, other times, it feels like everything goes haywire. What are your experiences? Do you have any rules of thumb for when to splurge on higher-quality reagents versus when to save a bit of money?
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